Strain-dependent variance in short-term adaptation effects of two xylose-fermenting strains of Saccharomyces cerevisiae

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Abstract

The limited tolerance of Saccharomyces cerevisiae to the inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. The aim of this study was to investigate the short-term adaptation effects in yeast strains with different genetic backgrounds. Fed-batch propagation cultures were supplemented with 40% wheat straw hydrolysate during the feed phase to adapt two different pentose-fermenting strains, CR01 and KE6-12. The harvested cells were used to inoculate fermentation media containing 80% or 90% wheat straw hydrolysate. The specific ethanol productivity during fermentation was up to 3.6 times higher for CR01 and 1.6 times higher for KE6-12 following adaptation. The influence of physiological parameters such as viability, storage carbohydrate content, and metabolite yields following short-term adaptation demonstrated that short-term adaptation was strain dependent.

Detaljer

Författare
  • Marlous van Dijk
  • Borbála Erdei
  • Mats Galbe
  • Yvonne Nygård
  • Lisbeth Olsson
Enheter & grupper
Externa organisationer
  • Chalmers Tekniska Högskola
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Bioenergi

Nyckelord

Originalspråkengelska
Artikelnummer121922
TidskriftBioresource Technology
Volym292
StatusPublished - 2019
PublikationskategoriForskning
Peer review utfördJa