Streptococcal Lancefield polysaccharides are critical cell wall determinants for human Group IIA secreted phospholipase A2 to exert its bactericidal effects

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift


Human Group IIA secreted phospholipase A2 (hGIIA) is an acute phase protein with bactericidal activity against Gram-positive bacteria. Infection models in hGIIA transgenic mice have suggested the importance of hGIIA as an innate defense mechanism against the human pathogens Group A Streptococcus (GAS) and Group B Streptococcus (GBS). Compared to other Gram-positive bacteria, GAS is remarkably resistant to hGIIA activity. To identify GAS resistance mechanisms, we exposed a highly saturated GAS M1 transposon library to recombinant hGIIA and compared relative mutant abundance with library input through transposon-sequencing (Tn-seq). Based on transposon prevalence in the output library, we identified nine genes, including dltA and lytR, conferring increased hGIIA susceptibility. In addition, seven genes conferred increased hGIIA resistance, which included two genes, gacH and gacI that are located within the Group A Carbohydrate (GAC) gene cluster. Using GAS 5448 wild-type and the isogenic gacI mutant and gacI-complemented strains, we demonstrate that loss of the GAC N-acetylglucosamine (GlcNAc) side chain in the ΔgacI mutant increases hGIIA resistance approximately 10-fold, a phenotype that is conserved across different GAS serotypes. Increased resistance is associated with delayed penetration of hGIIA through the cell wall. Correspondingly, loss of the Lancefield Group B Carbohydrate (GBC) rendered GBS significantly more resistant to hGIIA-mediated killing. This suggests that the streptococcal Lancefield antigens, which are critical determinants for streptococcal physiology and virulence, are required for the bactericidal enzyme hGIIA to exert its bactericidal function.


  • Vincent P. van Hensbergen
  • Elin Movert
  • Vincent de Maat
  • Christian Lüchtenborg
  • Yoann Le Breton
  • Gérard Lambeau
  • Christine Payré
  • Anna Henningham
  • Victor Nizet
  • Jos A.G. van Strijp
  • Britta Brügger
  • Fredric Carlsson
  • Kevin S. McIver
  • Nina M. van Sorge
Enheter & grupper
Externa organisationer
  • University Medical Center Utrecht
  • Heidelberg University
  • University of Maryland
  • University of California, San Diego
  • Utrecht University
  • University of Côte d'Azur

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området
Sidor (från-till)e1007348
TidskriftPLoS Pathogens
StatusPublished - 2018
Peer review utfördJa

Relaterad forskningsoutput

Elin Movert, 2019, Lund: Lund University, Faculty of Medicine. 85 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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