Streptolysin O induces the ubiquitination and degradation of pro-IL-1β

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Group A Streptococcus (GAS) is a common and versatile human pathogen causing a variety of diseases. One of the many virulence factors of GAS is the secreted pore-forming cytotoxin streptolysin O (SLO), which has been ascribed multiple properties, including inflammasome activation leading to release of the potent inflammatory cytokine IL-1β from infected macrophages. IL-1β is synthesized as an inactive pro-form, which is activated intracellularly through proteolytic cleavage. Here, we use a macrophage infection model to show that SLO specifically induces ubiquitination and degradation of pro-IL-1β. Ubiquitination was dependent on SLO being released from the infecting bacterium, and pore formation by SLO was required but not sufficient for the induction of ubiquitination. Our data provide evidence for a novel SLO-mediated mechanism of immune regulation, emphasizing the importance of this pore-forming toxin in bacterial virulence and pathogenesis.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Boston Children's Hospital
  • Norwegian University of Science and Technology
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Infektionsmedicin

Nyckelord

Originalspråkengelska
TidskriftJournal of Innate Immunity
StatusE-pub ahead of print - 2019
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Elsa Westerlund, 2019, Lund: Lund University, Faculty of Medicine. 84 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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