Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1

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Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. / Busenlehner, L S; Codreanu, S G; Holm, P J; Bhakat, P; Hebert, Hans; Morgenstern, R; Armstrong, R N.

I: Biochemistry, Vol. 43, Nr. 35, 2004, s. 11145-11152.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Busenlehner, LS, Codreanu, SG, Holm, PJ, Bhakat, P, Hebert, H, Morgenstern, R & Armstrong, RN 2004, 'Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1', Biochemistry, vol. 43, nr. 35, s. 11145-11152. https://doi.org/10.1021/bi048716k

APA

Busenlehner, L. S., Codreanu, S. G., Holm, P. J., Bhakat, P., Hebert, H., Morgenstern, R., & Armstrong, R. N. (2004). Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. Biochemistry, 43(35), 11145-11152. https://doi.org/10.1021/bi048716k

CBE

Busenlehner LS, Codreanu SG, Holm PJ, Bhakat P, Hebert H, Morgenstern R, Armstrong RN. 2004. Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. Biochemistry. 43(35):11145-11152. https://doi.org/10.1021/bi048716k

MLA

Vancouver

Busenlehner LS, Codreanu SG, Holm PJ, Bhakat P, Hebert H, Morgenstern R et al. Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. Biochemistry. 2004;43(35):11145-11152. https://doi.org/10.1021/bi048716k

Author

Busenlehner, L S ; Codreanu, S G ; Holm, P J ; Bhakat, P ; Hebert, Hans ; Morgenstern, R ; Armstrong, R N. / Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. I: Biochemistry. 2004 ; Vol. 43, Nr. 35. s. 11145-11152.

RIS

TY - JOUR

T1 - Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1

AU - Busenlehner, L S

AU - Codreanu, S G

AU - Holm, P J

AU - Bhakat, P

AU - Hebert, Hans

AU - Morgenstern, R

AU - Armstrong, R N

PY - 2004

Y1 - 2004

N2 - Microsomal glutathione (GSH) transferase 1 (MGST1) is a trimeric, integral membrane protein involved in cellular response to chemical or oxidative stress. The cytosolic domain of MGST1 harbors the GSH binding site and a cysteine residue (C49) that acts as a sensor of oxidative and chemical stress. Spatially resolved changes in the kinetics of backbone amide H/D exchange reveal that the binding of a single molecule of GSH/trimer induces a cooperative conformational transition involving movements of the transmembrane helices and a reordering of the cytosolic domain. Alkylation of the stress sensor preorganizes the helices and facilitates the cooperative transition resulting in catalytic activation.

AB - Microsomal glutathione (GSH) transferase 1 (MGST1) is a trimeric, integral membrane protein involved in cellular response to chemical or oxidative stress. The cytosolic domain of MGST1 harbors the GSH binding site and a cysteine residue (C49) that acts as a sensor of oxidative and chemical stress. Spatially resolved changes in the kinetics of backbone amide H/D exchange reveal that the binding of a single molecule of GSH/trimer induces a cooperative conformational transition involving movements of the transmembrane helices and a reordering of the cytosolic domain. Alkylation of the stress sensor preorganizes the helices and facilitates the cooperative transition resulting in catalytic activation.

U2 - 10.1021/bi048716k

DO - 10.1021/bi048716k

M3 - Article

VL - 43

SP - 11145

EP - 11152

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 35

ER -