Structural analysis of 2D-gel-separated glycoproteins from human cerebrospinal fluid by tandem high-resolution mass spectrometry

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

Detaljer

Författare
  • Kristina Håkansson
  • Mark R Emmett
  • Alan G Marshall
  • Pia Davidsson
  • Carol L Nilsson
Externa organisationer
  • Göteborgs universitet
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)581-8
Antal sidor8
TidskriftJournal of Proteome Research
Volym2
Utgåva nummer6
StatusPublished - 2003 dec 25
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa