Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme.

Detaljer

Författare
  • Sigurbjörg Rutardottir
  • Elena Karnaukhova
  • Chanin Nantasenamat
  • Napat Songtawee
  • Virapong Prachayasittikul
  • Mohsen Rajabi
  • Lena Wester Rosenlöf
  • Abdu I Alayash
  • Bo Åkerström
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)29-41
TidskriftBiochimica et Biophysica Acta - Proteins and Proteomics
Volym1864
Utgivningsnummer1
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa