Structural basis for the autoinhibition of the C-terminal kinase domain of human RSK1

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

p90 ribosomal S6 kinases (RSKs) respond to various mitogen stimuli and comprise two distinct protein kinase domains. The C-terminal kinase domain (CTKD) receives signal from ERK1/2 and adopts an autoinhibitory mechanism. Here, the crystal structure of human RSK1 CTKD is reported at 2.7 Å resolution. The structure shows a standard kinase fold, with the catalytic residues in the ATP-binding cleft orientated in optimal conformations for phosphotransfer. The inactivation of the CTKD is conferred by an extra α-helix (αL), which occupies the substrate-binding groove. In combination with previous knowledge, this structure indicates that activation of RSK1 involves the removal of αL from the substrate-binding groove induced by ERK1/2 phosphorylation.

Detaljer

Författare
  • Dan Li
  • Tian Min Fu
  • Jie Nan
  • Cong Liu
  • Lan Fen Li
  • Xiao Dong Su
Externa organisationer
  • Peking Union Medical College
  • Peking University
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)680-5
Antal sidor6
TidskriftActa Crystallographica. Section D: Biological Crystallography
Volym68
Utgåva nummerPt 6
StatusPublished - 2012 jun
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa