Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.

Detaljer

Författare
  • U H Stenman
  • E Paus
  • W J Allard
  • I Andersson
  • C Andres
  • T R Barnett
  • A Belenky
  • L Bellanger
  • C M Pellegrino
  • O P Bormer
  • G Davis
  • B Dowell
  • L S Grauer
  • D C Jette
  • B Karlsson
  • F T Kreutz
  • T M van der Kwast
  • L Lauren
  • M Leinimaa
  • J Leinonen
  • H J Linton
  • M Nap
  • O Nilsson
  • P C Ng
  • K Nustad
  • A Peter
  • K Pettersson
  • T Piironen
  • J Rapp
  • H G Rittenhouse
  • P D Rye
  • P Seguin
  • J Slota
  • R L Sokoloff
  • M R Suresh
  • D L Very
  • T J Wang
  • Ingrid Wigheden
  • R L Wolfert
  • K K Yeung
  • W-M Zhang
  • Z Zhou
  • J Hilgers
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1-12
TidskriftTumor Biology
Volym20
UtgivningsnummerSuppl. 1
StatusPublished - 1999
PublikationskategoriForskning
Peer review utfördJa