Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors

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Bibtex

@article{6ee7e7d8898d4d53897dabfa008a71e0,
title = "Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors",
abstract = "Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases.",
keywords = "endothelial growth factor, vascular, gene delivery, serotype 1, adeno-associated virus, muscle, ischemia",
author = "H Yan and YH Guo and P Zhang and LY Zu and XY Dong and L Chen and JW Tian and Xiaolong Fan and NP Wang and XB Wu and W Gao",
year = "2005",
doi = "10.1016/j.bbrc.2005.08.066",
language = "English",
volume = "336",
pages = "287--298",
journal = "Biochemical and Biophysical Research Communications",
issn = "1090-2104",
publisher = "Elsevier",
number = "1",

}