Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism

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Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism. / Podgórska, Anna; Ostaszewska-Bugajska, Monika; Borysiuk, Klaudia; Tarnowska, Agata; Jakubiak, Monika; Burian, Maria; Rasmusson, Allan G.; Szal, Bożena.

I: International Journal of Molecular Sciences, Vol. 19, Nr. 5, 1412, 09.05.2018.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Podgórska, Anna ; Ostaszewska-Bugajska, Monika ; Borysiuk, Klaudia ; Tarnowska, Agata ; Jakubiak, Monika ; Burian, Maria ; Rasmusson, Allan G. ; Szal, Bożena. / Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism. I: International Journal of Molecular Sciences. 2018 ; Vol. 19, Nr. 5.

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TY - JOUR

T1 - Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism

AU - Podgórska, Anna

AU - Ostaszewska-Bugajska, Monika

AU - Borysiuk, Klaudia

AU - Tarnowska, Agata

AU - Jakubiak, Monika

AU - Burian, Maria

AU - Rasmusson, Allan G.

AU - Szal, Bożena

PY - 2018/5/9

Y1 - 2018/5/9

N2 - Environmental stresses, including ammonium (NH4 +) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4 +-fed plants. Most plant species grown with NH4 + as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH4 + treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH4 +. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH4 + treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH4 +-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.

AB - Environmental stresses, including ammonium (NH4 +) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4 +-fed plants. Most plant species grown with NH4 + as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH4 + treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH4 +. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH4 + treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH4 +-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.

KW - Ammonium toxicity

KW - External type II NADPH dehydrogenase

KW - Glutathione metabolism

KW - Reactive oxygen species

KW - Redox homeostasis

UR - http://www.scopus.com/inward/record.url?scp=85046792909&partnerID=8YFLogxK

U2 - 10.3390/ijms19051412

DO - 10.3390/ijms19051412

M3 - Article

VL - 19

JO - International Journal of Molecular Sciences

T2 - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 5

M1 - 1412

ER -