Surface-modified nanoerythrosomes for potential optical imaging diagnostics

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Nanoerythrosomes (NERs), vesicle-like nanoparticles derived from red blood cells, represent a new and interesting vector for therapeutic molecules and imaging probes, mainly thanks to their high stability and excellent biocompatibility. Aiming to present a proof-of-concept of the use of NERs as diagnostic tools for in vitro/in vivo imaging purposes, we report here several functionalization routes to decorate the surfaces of NERs derived from bovine blood with two different fluorophores: 7-amino-4-methylcumarin and dibenzocyclooctinecyanine5.5. Notably, the fluorophores were cross-linked to the NERs surface with glutaraldehyde and, in the case of dibenzocyclooctinecyanine5.5, also using a click-chemistry route, termed strain-promoted azide-alkyne cycloaddition. The physicochemical characterization highlighted the high stability of the NERs derivatives in physiological conditions. Furthermore, the loading efficiency of the fluorophores on the NERs surface was evaluated using both UV–Vis spectroscopy and fluorescence microscopy.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University of Cagliari
  • Research Center for Colloids and Nanoscience
  • AdventHealth
  • University of Maryland
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biomedicinsk laboratorievetenskap/teknologi
  • Nanoteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)246-253
Antal sidor8
TidskriftJournal of Colloid and Interface Science
Volym582
Utgåva nummerPart A
StatusPublished - 2021
PublikationskategoriForskning
Peer review utfördJa