Surfactant-DNA gel particles: Formation and release characteristics

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Surfactant-DNA gel particles: Formation and release characteristics. / Moran, M. Carmen; Miguel, M. Graca; Lindman, Björn.

I: Biomacromolecules, Vol. 8, Nr. 12, 2007, s. 3886-3892.

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Moran, M. Carmen ; Miguel, M. Graca ; Lindman, Björn. / Surfactant-DNA gel particles: Formation and release characteristics. I: Biomacromolecules. 2007 ; Vol. 8, Nr. 12. s. 3886-3892.

RIS

TY - JOUR

T1 - Surfactant-DNA gel particles: Formation and release characteristics

AU - Moran, M. Carmen

AU - Miguel, M. Graca

AU - Lindman, Björn

PY - 2007

Y1 - 2007

N2 - Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/ deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA.

AB - Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/ deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA.

U2 - 10.1021/bm700850z

DO - 10.1021/bm700850z

M3 - Article

VL - 8

SP - 3886

EP - 3892

JO - Biomacromolecules

T2 - Biomacromolecules

JF - Biomacromolecules

SN - 1526-4602

IS - 12

ER -