Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-l-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9mg of Protein G per g of the cryogel.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Hacettepe University
  • Indienz AB
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strukturbiologi
Originalspråkengelska
Sidor (från-till)204-212
TidskriftJournal of Chromatography. B
Volym1021
Tidigt onlinedatum2016 jan 4
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa