T granules in human platelets function in TLR9 organization and signaling

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T granules in human platelets function in TLR9 organization and signaling. / Thon, Jonathan N; Peters, Christopher G; Machlus, Kellie R; Aslam, Rukhsana; Rowley, Jesse; Macleod, Hannah; Devine, Matthew T; Fuchs, Tobias A; Weyrich, Andrew S; Semple, John W; Flaumenhaft, Robert; Italiano, Joseph E.

I: Journal of Cell Biology, Vol. 198, Nr. 4, 20.08.2012, s. 561-74.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Thon, JN, Peters, CG, Machlus, KR, Aslam, R, Rowley, J, Macleod, H, Devine, MT, Fuchs, TA, Weyrich, AS, Semple, JW, Flaumenhaft, R & Italiano, JE 2012, 'T granules in human platelets function in TLR9 organization and signaling', Journal of Cell Biology, vol. 198, nr. 4, s. 561-74. https://doi.org/10.1083/jcb.201111136

APA

Thon, J. N., Peters, C. G., Machlus, K. R., Aslam, R., Rowley, J., Macleod, H., Devine, M. T., Fuchs, T. A., Weyrich, A. S., Semple, J. W., Flaumenhaft, R., & Italiano, J. E. (2012). T granules in human platelets function in TLR9 organization and signaling. Journal of Cell Biology, 198(4), 561-74. https://doi.org/10.1083/jcb.201111136

CBE

Thon JN, Peters CG, Machlus KR, Aslam R, Rowley J, Macleod H, Devine MT, Fuchs TA, Weyrich AS, Semple JW, Flaumenhaft R, Italiano JE. 2012. T granules in human platelets function in TLR9 organization and signaling. Journal of Cell Biology. 198(4):561-74. https://doi.org/10.1083/jcb.201111136

MLA

Vancouver

Thon JN, Peters CG, Machlus KR, Aslam R, Rowley J, Macleod H et al. T granules in human platelets function in TLR9 organization and signaling. Journal of Cell Biology. 2012 aug 20;198(4):561-74. https://doi.org/10.1083/jcb.201111136

Author

Thon, Jonathan N ; Peters, Christopher G ; Machlus, Kellie R ; Aslam, Rukhsana ; Rowley, Jesse ; Macleod, Hannah ; Devine, Matthew T ; Fuchs, Tobias A ; Weyrich, Andrew S ; Semple, John W ; Flaumenhaft, Robert ; Italiano, Joseph E. / T granules in human platelets function in TLR9 organization and signaling. I: Journal of Cell Biology. 2012 ; Vol. 198, Nr. 4. s. 561-74.

RIS

TY - JOUR

T1 - T granules in human platelets function in TLR9 organization and signaling

AU - Thon, Jonathan N

AU - Peters, Christopher G

AU - Machlus, Kellie R

AU - Aslam, Rukhsana

AU - Rowley, Jesse

AU - Macleod, Hannah

AU - Devine, Matthew T

AU - Fuchs, Tobias A

AU - Weyrich, Andrew S

AU - Semple, John W

AU - Flaumenhaft, Robert

AU - Italiano, Joseph E

PY - 2012/8/20

Y1 - 2012/8/20

N2 - Human and murine platelets (PLTs) variably express toll-like receptors (TLRs), which link the innate and adaptive immune responses during infectious inflammation and atherosclerotic vascular disease. In this paper, we show that the TLR9 transcript is specifically up-regulated during pro-PLT production and is distributed to a novel electron-dense tubular system-related compartment we have named the T granule. TLR9 colocalizes with protein disulfide isomerase and is associated with either VAMP 7 or VAMP 8, which regulates its distribution in PLTs on contact activation (spreading). Preincubation of PLTs with type IV collagen specifically increased TLR9 and CD62P surface expression and augmented oligodeoxynucleotide (ODN) sequestration and PLT clumping upon addition of bacterial/viral ODNs. Collectively, this paper (a) tracks TLR9 to a new intracellular compartment in PLTs and (b) describes a novel mechanism of TLR9 organization and signaling in human PLTs.

AB - Human and murine platelets (PLTs) variably express toll-like receptors (TLRs), which link the innate and adaptive immune responses during infectious inflammation and atherosclerotic vascular disease. In this paper, we show that the TLR9 transcript is specifically up-regulated during pro-PLT production and is distributed to a novel electron-dense tubular system-related compartment we have named the T granule. TLR9 colocalizes with protein disulfide isomerase and is associated with either VAMP 7 or VAMP 8, which regulates its distribution in PLTs on contact activation (spreading). Preincubation of PLTs with type IV collagen specifically increased TLR9 and CD62P surface expression and augmented oligodeoxynucleotide (ODN) sequestration and PLT clumping upon addition of bacterial/viral ODNs. Collectively, this paper (a) tracks TLR9 to a new intracellular compartment in PLTs and (b) describes a novel mechanism of TLR9 organization and signaling in human PLTs.

KW - Animals

KW - Atherosclerosis

KW - Blood Platelets

KW - Cell Compartmentation

KW - Cytoplasmic Granules

KW - Erythroid Precursor Cells

KW - Humans

KW - Mice

KW - Platelet Activation

KW - Signal Transduction

KW - Thrombopoiesis

KW - Toll-Like Receptor 9

KW - Up-Regulation

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1083/jcb.201111136

DO - 10.1083/jcb.201111136

M3 - Article

C2 - 22908309

VL - 198

SP - 561

EP - 574

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -