Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

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Bibtex

@article{926c17d5e0404b0cb26444788ebfdec3,
title = "Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding",
abstract = "During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41iCre/iCre knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41iCre/+ mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41iCre/iCre sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41iCre/iCre sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation.",
keywords = "Beta-defensin, Epididymis, Flagellar motility pattern, ICre knock-in, Sperm maturation, Sperm-oocyte binding",
author = "Ida Bj{\"o}rkgren and Luis Alvarez and Nelli Blank and Melanie Balbach and Heikki Turunen and Laajala, {Teemu Daniel} and Jussi Toivanen and Anton Krutskikh and Niklas Wahlberg and Ilpo Huhtaniemi and Matti Poutanen and Dagmar Wachten and Petra Sipil{\"a}",
year = "2016",
month = "5",
day = "15",
doi = "10.1016/j.mce.2016.03.013",
language = "English",
volume = "427",
pages = "143--154",
journal = "Molecular and Cellular Endocrinology",
issn = "1872-8057",
publisher = "Elsevier",

}