Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding. / Björkgren, Ida; Alvarez, Luis; Blank, Nelli; Balbach, Melanie; Turunen, Heikki; Laajala, Teemu Daniel; Toivanen, Jussi; Krutskikh, Anton; Wahlberg, Niklas; Huhtaniemi, Ilpo; Poutanen, Matti; Wachten, Dagmar; Sipilä, Petra.

I: Molecular and Cellular Endocrinology, Vol. 427, 15.05.2016, s. 143-154.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Björkgren, I, Alvarez, L, Blank, N, Balbach, M, Turunen, H, Laajala, TD, Toivanen, J, Krutskikh, A, Wahlberg, N, Huhtaniemi, I, Poutanen, M, Wachten, D & Sipilä, P 2016, 'Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding', Molecular and Cellular Endocrinology, vol. 427, s. 143-154. https://doi.org/10.1016/j.mce.2016.03.013

APA

CBE

Björkgren I, Alvarez L, Blank N, Balbach M, Turunen H, Laajala TD, Toivanen J, Krutskikh A, Wahlberg N, Huhtaniemi I, Poutanen M, Wachten D, Sipilä P. 2016. Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding. Molecular and Cellular Endocrinology. 427:143-154. https://doi.org/10.1016/j.mce.2016.03.013

MLA

Vancouver

Author

Björkgren, Ida ; Alvarez, Luis ; Blank, Nelli ; Balbach, Melanie ; Turunen, Heikki ; Laajala, Teemu Daniel ; Toivanen, Jussi ; Krutskikh, Anton ; Wahlberg, Niklas ; Huhtaniemi, Ilpo ; Poutanen, Matti ; Wachten, Dagmar ; Sipilä, Petra. / Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding. I: Molecular and Cellular Endocrinology. 2016 ; Vol. 427. s. 143-154.

RIS

TY - JOUR

T1 - Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding

AU - Björkgren, Ida

AU - Alvarez, Luis

AU - Blank, Nelli

AU - Balbach, Melanie

AU - Turunen, Heikki

AU - Laajala, Teemu Daniel

AU - Toivanen, Jussi

AU - Krutskikh, Anton

AU - Wahlberg, Niklas

AU - Huhtaniemi, Ilpo

AU - Poutanen, Matti

AU - Wachten, Dagmar

AU - Sipilä, Petra

PY - 2016/5/15

Y1 - 2016/5/15

N2 - During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41iCre/iCre knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41iCre/+ mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41iCre/iCre sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41iCre/iCre sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation.

AB - During epididymal maturation, sperm acquire the ability to swim progressively by interacting with proteins secreted by the epididymal epithelium. Beta-defensin proteins, expressed in the epididymis, continue to regulate sperm motility during capacitation and hyperactivation in the female reproductive tract. We characterized the mouse beta-defensin 41 (DEFB41), by generating a mouse model with iCre recombinase inserted into the first exon of the gene. The homozygous Defb41iCre/iCre knock-in mice lacked Defb41 expression and displayed iCre recombinase activity in the principal cells of the proximal epididymis. Heterozygous Defb41iCre/+ mice can be used to generate epididymis specific conditional knock-out mouse models. Homozygous Defb41iCre/iCre sperm displayed a defect in sperm motility with the flagella primarily bending in the pro-hook conformation while capacitated wild-type sperm more often displayed the anti-hook conformation. This led to a reduced straight line motility of Defb41iCre/iCre sperm and weaker binding to the oocyte. Thus, DEFB41 is required for proper sperm maturation.

KW - Beta-defensin

KW - Epididymis

KW - Flagellar motility pattern

KW - ICre knock-in

KW - Sperm maturation

KW - Sperm-oocyte binding

U2 - 10.1016/j.mce.2016.03.013

DO - 10.1016/j.mce.2016.03.013

M3 - Article

VL - 427

SP - 143

EP - 154

JO - Molecular and Cellular Endocrinology

T2 - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 1872-8057

ER -