The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - A yeast of biotechnological interest

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Abstract

Background: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. Results: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. Conclusions: The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.

Detaljer

Författare
  • LaBRI, University Bordeaux 1, France
Externa organisationer
  • Leibniz Institute of Plant Genetics and Crop Plant Research
  • AgroParisTech - Engref Centre de Nancy
  • INRA National Institute of Agricultural Research
  • Agricultural University of Krakow
  • Center for Genomic Regulation
  • Pompeu Fabra University
  • University of Strasbourg
  • Aix-Marseille University
  • CEA, Saclay Biology and Technologies Institute (IBiTec-S)
  • European Commission
  • Centre National de Génotypage
  • Canterbury University
  • Ernst-Moritz-Arndt University Greifswald
  • University of Paris-Sud
  • International Institute of Molecular and Cell Biology, Warsaw
  • Université d'Évry Val-d'Essonne
  • Pasteur Institute
  • University of Lyon
  • Institut de Biologie de l'École Normale Supérieure (IBENS)
  • University of Bordeaux
  • National Institute for Research in Computer Science and Control (INIRA)
Forskningsområden

Nyckelord

Originalspråkengelska
Artikelnummer66
TidskriftBiotechnology for Biofuels
Volym7
Utgivningsnummer1
StatusPublished - 2014 apr 24
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa