The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

Detaljer

Författare
  • Hans Elmlund
  • Vera Baraznenok
  • Martin Lindahl
  • Camilla O. Samuelsen
  • Philip J. B. Koeck
  • Steen Holmberg
  • Hans Hebert
  • Claes M. Gustafsson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)15788-15793
TidskriftProceedings of the National Academy of Sciences
Volym103
Utgivningsnummer43
StatusPublished - 2006
PublikationskategoriForskning
Peer review utfördJa