The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE(+) cells in memory responses is particularly unclear. IgE(+) B cell differentiation is characterized by a transient GC phase, a bias toward the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE(+) GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B cell receptor function and increased apoptosis. IgE(+) GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are linked to distinct B cell differentiation fates: direct switching generates IgE(+) GC cells, whereas sequential switching gives rise to IgE(+) PCs. We propose a comprehensive model for the generation and memory of IgE responses.

Detaljer

Författare
  • Jin-Shu He
  • Michael Meyer-Hermann
  • Deng Xiangying
  • Lim Yok Zuan
  • Leigh Ann Jones
  • Lakshmi Ramakrishna
  • Victor C de Vries
  • Jayashree Dolpady
  • Hoi Aina
  • Sabrina Joseph
  • Sriram Narayanan
  • Sharrada Subramaniam
  • Manoj Puthia
  • Glenn Wong
  • Huizhong Xiong
  • Michael Poidinger
  • Joseph F Urban
  • Juan J Lafaille
  • Maria A Curotto de Lafaille
Externa organisationer
  • Singapore Immunology Network
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)2755-2771
TidskriftJournal of Experimental Medicine
Volym210
Utgåva nummer12
StatusPublished - 2013 nov 18
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa