The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p

Detaljer

Författare
  • N. Clyne
  • R. Wibom
  • N. Havu
  • E. Hultman
  • S. K. Pehrsson
  • B. Persson
Externa organisationer
  • Karolinska University Hospital
  • Stockholms universitet
  • Karolinska Institute
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)153-159
Antal sidor7
TidskriftScandinavian Journal of Clinical & Laboratory Investigation
Volym50
Utgivningsnummer2
StatusPublished - 1990
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa