The effect of PAMAM G6 dendrimers on the structure of lipid vesicles

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. PAMAM dendrimers, in particular, have been widely investigated and are efficiently translocated into the cell. The mechanism of translocation, however, is still unknown. Recently it was proposed that PAMAM dendrimers are able to open holes in lipid bilayers by stealing lipid from the bilayer and forming "dendrisomes''. The present work intends to contribute in the clarification of this question: why are dendrimers able to translocate into the cell? We create simple models for cell membranes by using small lipid vesicles that present a single lipid phase at physiologically relevant conditions. We then follow the effect that dendrimers have on the structure of the vesicles by using a combination of various techniques: dynamic light scattering, cryo-TEM and small angle X-ray scattering. We discuss our results with respect to the previous findings and reflect on their possible implications for real translocation in living cells.

Detaljer

Författare
  • Anna Akesson
  • Kristian Moss Bendtsen
  • Manja A. Beherens
  • Jan Skov Pedersen
  • Viveka Alfredsson
  • Marite Cardenas Gomez
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
Originalspråkengelska
Sidor (från-till)12267-12272
TidskriftPhysical Chemistry Chemical Physics
Volym12
Utgåva nummer38
StatusPublished - 2010
PublikationskategoriForskning
Peer review utfördJa