The effects of platelet apheresis in total hip replacement surgery on platelet activation

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.

Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.

Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.

Results and conclusions: A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1–23% in peroperative activation. PRP harvest did not significantly alter platelet activity.

The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value ± SD 86% ± 7.5%).

Detaljer

Författare
  • G Ekbäck
  • B Edlund
  • A Smolowics
  • K Axelsson
  • J Kjellberg
  • Ulf Schött
Externa organisationer
  • Halmstad County Hospital
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Anestesi och intensivvård
Originalspråkengelska
Sidor (från-till)68-73
TidskriftActa Anaesthesiologica Scandinavica
Volym46
Utgåva nummer1
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa

Related projects

Ulf Schött

1990/05/012019/03/31

Projekt: Forskning

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