The growth of external AM fungal mycelium in sand dunes and in experimental systems

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The growth of external AM fungal mycelium in sand dunes and in experimental systems. / Olsson, Pål Axel; Wilhelmsson, P.

I: Plant and Soil, Vol. 226, Nr. 2, 2000, s. 161-169.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - The growth of external AM fungal mycelium in sand dunes and in experimental systems

AU - Olsson, Pål Axel

AU - Wilhelmsson, P

PY - 2000

Y1 - 2000

N2 - We estimated the biomass and growth of arbuscular mycorrhizal (AM) mycelium in sand dunes using signature fatty acids. Mesh bags and tubes, containing initially mycelium-free sand, were buried in the field near the roots of the dune grass Ammophila arenaria L. AM fungal mycelia were detected at a distance of about 8.5 cm from the roots after 68 days of growth by use of neutral lipid fatty acid (NLFA) 16:15. The average rate of mycelium extension during September and October was estimated as 1.2 mm day–1. The lipid and fatty acid compositions of AM fungal mycelia of isolates and from sand dunes were analysed and showed all to be of a similar composition. Phospholipid fatty acids (PLFAs) can be used as indicators of microbial biomass. The mycelium of G. intraradices growing in glass beads contained 8.3 nmol PLFAs per mg dry biomass, and about 15% of the PLFAs in G. intraradices, G. claroideum and AM fungal mycelium extracted from sand dunes, consisted of the signature PLFA 16:15. We thus suggest a conversion factor of 1.2 nmol PLFA 16:15 per mg dry biomass. Calculations using this conversion factor indicated up to 34 g dry AM fungal biomass per g sand in the sand dunes, which was less than one tenth of that found in an experimental system with Glomus spp. growing with cucumber as plant associate in agricultural soil. The PLFA results from different systems indicated that the biomass of the AM fungi constitutes a considerable part of the total soil microbial biomass. Calculations based on ATP of AM fungi in an experimental growth system indicated that the biomass of the AM fungi constituted approximately 30% of the total microbial biomass.

AB - We estimated the biomass and growth of arbuscular mycorrhizal (AM) mycelium in sand dunes using signature fatty acids. Mesh bags and tubes, containing initially mycelium-free sand, were buried in the field near the roots of the dune grass Ammophila arenaria L. AM fungal mycelia were detected at a distance of about 8.5 cm from the roots after 68 days of growth by use of neutral lipid fatty acid (NLFA) 16:15. The average rate of mycelium extension during September and October was estimated as 1.2 mm day–1. The lipid and fatty acid compositions of AM fungal mycelia of isolates and from sand dunes were analysed and showed all to be of a similar composition. Phospholipid fatty acids (PLFAs) can be used as indicators of microbial biomass. The mycelium of G. intraradices growing in glass beads contained 8.3 nmol PLFAs per mg dry biomass, and about 15% of the PLFAs in G. intraradices, G. claroideum and AM fungal mycelium extracted from sand dunes, consisted of the signature PLFA 16:15. We thus suggest a conversion factor of 1.2 nmol PLFA 16:15 per mg dry biomass. Calculations using this conversion factor indicated up to 34 g dry AM fungal biomass per g sand in the sand dunes, which was less than one tenth of that found in an experimental system with Glomus spp. growing with cucumber as plant associate in agricultural soil. The PLFA results from different systems indicated that the biomass of the AM fungi constitutes a considerable part of the total soil microbial biomass. Calculations based on ATP of AM fungi in an experimental growth system indicated that the biomass of the AM fungi constituted approximately 30% of the total microbial biomass.

U2 - 10.1023/A:1026565314345

DO - 10.1023/A:1026565314345

M3 - Article

VL - 226

SP - 161

EP - 169

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 2

ER -