The purification of membranes by affinity partitioning

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We describe affinity partitioning as a preparative method for membranes and membraneous structures such as organelles, cells, and viruses. Biospecific affinity partitioning is carried out in aqueous polymer two- phase systems, commonly with polyethylene glycol and dextran as phase polymers, in an environment compatible with membrane structures. Ideally, two-phase conditions are chosen to partition the bulk of membrane material into one phase, while the affinity ligand, conjugated to the second phase polymer, will selectively pull the membranes to be isolated into this phase. Suitable ligands include lectins, antibodies, and receptor-specific agents. Because the method has so far been used successfully in rather few instances, all using high ligand receptor densities in target membranes, the discussion focuses on factors to be considered when developing affinity partitioning conditions using new ligands.

Detaljer

Författare
  • A Persson
  • Bengt Jergil
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)1304-1310
TidskriftFASEB Journal
Volym9
Utgåva nummer13
StatusPublished - 1995
PublikationskategoriForskning
Peer review utfördJa