The use of dispersed pancreatic islet cells in measurements of transmembrane transport

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.

Detaljer

Författare
Externa organisationer
  • Umeå University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Endokrinologi och diabetes
Originalspråkengelska
Sidor (från-till)73-79
Antal sidor7
TidskriftAnalytical Biochemistry
Volym63
Utgåva nummer1
StatusPublished - 1975 jan 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa