Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD2), we further explored its role in thermoregulation. Our study revealed that rising temperature of POA alters the activity of Ptgds-expressing neurons so as to increase PGD2 production. PGD2 activates its receptor DP1 and excites downstream neurons in the ventral medial preoptic area (vMPO) that mediates body temperature decrease, a negative feedback loop for thermoregulation.

Detaljer

Författare
  • Tongfei A Wang
  • Chin Fen Teo
  • Malin Åkerblom
  • Chao Chen
  • Marena Tynan-La Fontaine
  • Vanille Juliette Greiner
  • Aaron Diaz
  • Michael T McManus
  • Yuh Nung Jan
  • Lily Y Jan
Externa organisationer
  • University of California, San Francisco
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper
Originalspråkengelska
Sidor (från-till)309-322.e7
TidskriftNeuron
Volym103
Utgivningsnummer2
StatusPublished - 2019 jul 17
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa

Bibliografisk information

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