Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We demonstrate an artificial three-dimensional (3D) electrical active human neuronal network system, by the growth of brain neural progenitors in highly porous low density electrospun poly-ε-caprolactone (PCL) fiber scaffolds. In neuroscience research cell-based assays are important experimental instruments for studying neuronal function in health and disease. Traditional cell culture at 2D-surfaces induces abnormal cell-cell contacts and network formation. Hence, there is a tremendous need to explore in vivo-resembling 3D neural cell culture approaches. We present an improved electrospinning method for fabrication of scaffolds that promote neuronal differentiation into highly 3D integrated networks, formation of inhibitory and excitatory synapses and extensive neurite growth. Notably, in 3D scaffolds in vivo-resembling intermixed neuronal and glial cell network were formed, whereas in parallel 2D cultures a neuronal cell layer grew separated from an underlying glial cell layer. Hence, the use of the 3D cell assay presented will most likely provide more physiological relevant results.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1563-1573
TidskriftNanomedicine: Nanotechnology, Biology, and Medicine
Volym13
Utgivningsnummer4
Tidigt onlinedatum2017 jan 5
StatusPublished - 2017
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Marina Castro Zalis, 2017, Lund: Lund University, Faculty of Medicine. 95 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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