Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.

Detaljer

Författare
  • Darcy E. Wagner
  • Nicholas R. Bonenfant
  • Dino Sokocevic
  • Michael J DeSarno
  • Zachary D. Borg
  • Charles S. Parsons
  • Elice M. Brooks
  • Joseph Platz
  • Zain I. Khalpey
  • David M. Hoganson
  • Bin Deng
  • Ying Wai Lam
  • Rachael A. Oldinski
  • Takamaru Ashikaga
  • Daniel J. Weiss
Externa organisationer
  • University of Vermont
  • University of Arizona
  • Washington University in St. Louis
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)2664-2679
TidskriftBiomaterials
Volym35
Utgivningsnummer9
StatusPublished - 2014 mar
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa