Towards industrial pentose-fermenting yeast strains

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Towards industrial pentose-fermenting yeast strains. / Hahn-Hägerdal, Bärbel; Karhumaa, Kaisa; Fonseca, Cesar; Spencer-Martins, Isabel; Gorwa-Grauslund, Marie-Francoise.

I: Applied Microbiology and Biotechnology, Vol. 74, Nr. 5, 2007, s. 937-953.

Forskningsoutput: TidskriftsbidragÖversiktsartikel

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Hahn-Hägerdal, Bärbel ; Karhumaa, Kaisa ; Fonseca, Cesar ; Spencer-Martins, Isabel ; Gorwa-Grauslund, Marie-Francoise. / Towards industrial pentose-fermenting yeast strains. I: Applied Microbiology and Biotechnology. 2007 ; Vol. 74, Nr. 5. s. 937-953.

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TY - JOUR

T1 - Towards industrial pentose-fermenting yeast strains

AU - Hahn-Hägerdal, Bärbel

AU - Karhumaa, Kaisa

AU - Fonseca, Cesar

AU - Spencer-Martins, Isabel

AU - Gorwa-Grauslund, Marie-Francoise

PY - 2007

Y1 - 2007

N2 - Production of bioethanol from forest and agricultural products requires a fermenting organism that converts all types of sugars in the raw material to ethanol in high yield and with a high rate. This review summarizes recent research aiming at developing industrial strains of Saccharomyces cerevisiae with the ability to ferment all lignocellulose-derived sugars. The properties required from the industrial yeast strains are discussed in relation to four benchmarks: (1) process water economy, (2) inhibitor tolerance, (3) ethanol yield, and (4) specific ethanol productivity. Of particular importance is the tolerance of the fermenting organism to fermentation inhibitors formed during fractionation/pretreatment and hydrolysis of the raw material, which necessitates the use of robust industrial strain background. While numerous metabolic engineering strategies have been developed in laboratory yeast strains, only a few approaches have been realized in industrial strains. The fermentation performance of the existing industrial pentose-fermenting S. cerevisiae strains in lignocellulose hydrolysate is reviewed. Ethanol yields of more than 0.4 g ethanol/g sugar have been achieved with several xylose-fermenting industrial strains such as TMB 3400, TMB 3006, and 424A(LNF-ST), carrying the heterologous xylose utilization pathway consisting of xylose reductase and xylitol dehydrogenase, which demonstrates the potential of pentose fermentation in improving lignocellulosic ethanol production.

AB - Production of bioethanol from forest and agricultural products requires a fermenting organism that converts all types of sugars in the raw material to ethanol in high yield and with a high rate. This review summarizes recent research aiming at developing industrial strains of Saccharomyces cerevisiae with the ability to ferment all lignocellulose-derived sugars. The properties required from the industrial yeast strains are discussed in relation to four benchmarks: (1) process water economy, (2) inhibitor tolerance, (3) ethanol yield, and (4) specific ethanol productivity. Of particular importance is the tolerance of the fermenting organism to fermentation inhibitors formed during fractionation/pretreatment and hydrolysis of the raw material, which necessitates the use of robust industrial strain background. While numerous metabolic engineering strategies have been developed in laboratory yeast strains, only a few approaches have been realized in industrial strains. The fermentation performance of the existing industrial pentose-fermenting S. cerevisiae strains in lignocellulose hydrolysate is reviewed. Ethanol yields of more than 0.4 g ethanol/g sugar have been achieved with several xylose-fermenting industrial strains such as TMB 3400, TMB 3006, and 424A(LNF-ST), carrying the heterologous xylose utilization pathway consisting of xylose reductase and xylitol dehydrogenase, which demonstrates the potential of pentose fermentation in improving lignocellulosic ethanol production.

U2 - 10.1007/s00253-006-0827-2

DO - 10.1007/s00253-006-0827-2

M3 - Review article

VL - 74

SP - 937

EP - 953

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 1432-0614

IS - 5

ER -