Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

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Abstract

A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.

Detaljer

Författare
  • Per Falås
  • Kevin S. Jewell
  • Nina Hermes
  • Arne Wick
  • Thomas A. Ternes
  • Adriano Joss
  • Jeppe Lund Nielsen
Enheter & grupper
Externa organisationer
  • Swiss Federal Institute of Aquatic Science and Technology
  • Federal Institute of Hydrology
  • Aalborg University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)405-416
Antal sidor12
TidskriftWater Research
Volym141
StatusPublished - 2018 sep 15
PublikationskategoriForskning
Peer review utfördJa