Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.

Detaljer

Författare
  • Maria Silow
  • Mikael Oliveberg
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)6084-6086
TidskriftProceedings of the National Academy of Sciences
Volym94
Utgivningsnummer12
StatusPublished - 1997
PublikationskategoriForskning
Peer review utfördJa