Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana. / Schagerlöf, Ulrika; Wilson, Greer; Hebert, Hans; Al-Karadaghi, Salam; Hägerhäll, Cecilia.

I: Plant Molecular Biology, Vol. 62, Nr. 1-2, 2006, s. 215-221.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

APA

CBE

MLA

Vancouver

Author

Schagerlöf, Ulrika ; Wilson, Greer ; Hebert, Hans ; Al-Karadaghi, Salam ; Hägerhäll, Cecilia. / Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana. I: Plant Molecular Biology. 2006 ; Vol. 62, Nr. 1-2. s. 215-221.

RIS

TY - JOUR

T1 - Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana

AU - Schagerlöf, Ulrika

AU - Wilson, Greer

AU - Hebert, Hans

AU - Al-Karadaghi, Salam

AU - Hägerhäll, Cecilia

PY - 2006

Y1 - 2006

N2 - Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.

AB - Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.

KW - gp91phox

KW - FRO2

KW - ferric-chelate reductases

KW - oxidases

KW - superoxide generating NADPH

KW - iron transport

KW - alkaline phosphatase

U2 - 10.1007/s11103-006-9015-0

DO - 10.1007/s11103-006-9015-0

M3 - Article

VL - 62

SP - 215

EP - 221

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 1573-5028

IS - 1-2

ER -