Treatment with anti-factor VIIa in acute pancreatitis in rats: Blocking both coagulation and inflammation?

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Objective. Acute pancreatitis starts as an autodigestive process restricted to the pancreas and progresses to a systemic inflammation via cytokine release into the blood stream. Several inhibitors of the coagulation cascade, including active- siteinactivated factor VIIa, have shown anti- inflammatory properties in other inflammatory models than acute pancreatitis. Free radical scavengers have proven useful in reducing the oxidative damage during hyperinflammatory conditions. The aim of this study was to investigate whether pretreatment with FVIIai would have any effect on the multiple organ dysfunction syndrome ( MODS) in severe acute pancreatitis. Material and methods. Experimental acute pancreatitis was induced by intraductal infusion of taurodeoxycholate in the pancreatic duct. The animals were pretreated with N- acetyl- cysteine and active- site- inactivated factor VIIa. Neutrophil infiltration in the lungs, ileum and colon was quantified by myeloperoxidase activity. Inflammatory markers, IL- 6 and MIP- 2, were measured using ELISA. Results. Tissue infiltration of neutrophils in the lungs, ileum and colon significantly increased during acute pancreatitis as compared to sham operation. These levels were reduced by pretreatment with N- acetylcysteine and active- site- inactivated factor VIIa. Levels of interleukin- 6 and macrophage inflammatory protein- 2 increased significantly during acute pancreatitis. Pretreatment with NAC and FVIIai reduced these levels. Conclusions. Both N- acetylcysteine and active- site- inactivated factor VIIa showed powerful antiinflammatory properties in experimental acute pancreatitis. As they exert their effects through different physiological mechanisms, they represent potential candidates for future multimodal treatment of acute pancreatitis.

Detaljer

Författare
  • Ellen Andersson
  • Jakob B Axelsson
  • Lars Christian Pedersen
  • Torben Elm
  • Roland Andersson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Gastroenterologi
Originalspråkengelska
Sidor (från-till)765-770
TidskriftScandinavian Journal of Gastroenterology
Volym42
Utgåva nummer6
StatusPublished - 2007
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Andersson, E., 2010, Ellen Andersson. 136 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Axelsson, J. B., 2008, Department of Clinical Sciences, Lund University. 121 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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