Turning vice into virtue: Using Batch-Effects to Detect Errors in Large Genomic Datasets

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

It is often unavoidable to combine data from different sequencing centers or sequencing platforms when compiling datasets with a large number of individuals. However, the different data are likely to contain specific systematic errors that will appear as SNPs. Here, we devise a method to detect systematic errors in combined datasetIs. To measure quality differences between individual genomes, we study pairs of variants that reside on different chromosomes and co-occur in individuals. The abundance of these pairs of variants in different genomes is then used to detect systematic errors due to batch effects. Applying our method to the 1000 Genomes dataset, we find that coding regions are enriched for errors, where about 1% of the higher-frequency variants are predicted to be erroneous, whereas errors outside of coding regions are much rarer (<0.001%).As expected, predicted errors are found less often than other variants in a dataset that was generated with a different sequencing technology, indicating that many of the candidates are indeed errors. However, predicted 1000 Genomes errors are also found in other large datasets; our observation is thus not specific to the 1000 Genomes dataset. Our results show that batch effects can be turned into a virtue by using the resulting variation in large scale datasets to detect systematic errors.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Max Planck Institute for Evolutionary Anthropology
  • Institute for Molecular Medicine Finland (FIMM)
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Genetik
  • Bioinformatik och systembiologi
Originalspråkengelska
Sidor (från-till)2697–2708
TidskriftGenome Biology and Evolution
Volym10
Utgivningsnummer10
StatusPublished - 2018 sep 10
PublikationskategoriForskning
Peer review utfördJa