Two novel IgG endopeptidases of Streptococcus equi

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Two novel IgG endopeptidases of Streptococcus equi. / Hulting, Greta; Flock, Margareta; Frykberg, Lars; Lannergård, Jonas; Flock, Jan-Ingmar; Guss, Bengt.

I: FEMS Microbiology Letters, Vol. 298, Nr. 1, 2009, s. 44-50.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Hulting, G, Flock, M, Frykberg, L, Lannergård, J, Flock, J-I & Guss, B 2009, 'Two novel IgG endopeptidases of Streptococcus equi', FEMS Microbiology Letters, vol. 298, nr. 1, s. 44-50. https://doi.org/10.1111/j.1574-6968.2009.01698.x

APA

Hulting, G., Flock, M., Frykberg, L., Lannergård, J., Flock, J-I., & Guss, B. (2009). Two novel IgG endopeptidases of Streptococcus equi. FEMS Microbiology Letters, 298(1), 44-50. https://doi.org/10.1111/j.1574-6968.2009.01698.x

CBE

Hulting G, Flock M, Frykberg L, Lannergård J, Flock J-I, Guss B. 2009. Two novel IgG endopeptidases of Streptococcus equi. FEMS Microbiology Letters. 298(1):44-50. https://doi.org/10.1111/j.1574-6968.2009.01698.x

MLA

Vancouver

Hulting G, Flock M, Frykberg L, Lannergård J, Flock J-I, Guss B. Two novel IgG endopeptidases of Streptococcus equi. FEMS Microbiology Letters. 2009;298(1):44-50. https://doi.org/10.1111/j.1574-6968.2009.01698.x

Author

Hulting, Greta ; Flock, Margareta ; Frykberg, Lars ; Lannergård, Jonas ; Flock, Jan-Ingmar ; Guss, Bengt. / Two novel IgG endopeptidases of Streptococcus equi. I: FEMS Microbiology Letters. 2009 ; Vol. 298, Nr. 1. s. 44-50.

RIS

TY - JOUR

T1 - Two novel IgG endopeptidases of Streptococcus equi

AU - Hulting, Greta

AU - Flock, Margareta

AU - Frykberg, Lars

AU - Lannergård, Jonas

AU - Flock, Jan-Ingmar

AU - Guss, Bengt

PY - 2009

Y1 - 2009

N2 - Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection.

AB - Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection.

KW - vaccine

KW - IgG endopeptidases

KW - IdeS

KW - IdeZ

KW - Streptococcus equi

KW - IdeE

U2 - 10.1111/j.1574-6968.2009.01698.x

DO - 10.1111/j.1574-6968.2009.01698.x

M3 - Article

VL - 298

SP - 44

EP - 50

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 1574-6968

IS - 1

ER -