Two subunits of the F0F1-ATPase are phosphorylated in the inner mitochondrial membrane

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Abstract

Inside-out submitochondrial particles from potato tuber mitochondria were incubated with [γ-32P]ATP. More than 16 phosphorylated polypeptides were detected by autoradiography on an SDS-gel. Two phosphoproteins, migrating at 22 and 28 kDa, were excised from the SDS-gel, electroeluted, and purified further by anion chromatography. The phosphoproteins were N-terminally sequenced. Over the regions sequenced, the 22 and 28 kDa phosphoproteins had 100% sequence identity with potato proteins identified as the δ'-subunit of the F1-ATPase and the b-subunit of the F0-ATPase, respectively. We suggest that phosphorylation of these proteins may control the interaction between F1 and F0 and regulate energy coupling in oxidative phosphorylation.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Lund University
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)664-668
TidskriftBiochemical and Biophysical Research Communications
Volym243
Utgåva nummer3
StatusPublished - 1998 feb 24
PublikationskategoriForskning
Peer review utfördJa