Two-photon excitation in fluorescence polarization receptor-ligand binding assay

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

Detaljer

Författare
  • M E Tirri
  • R J Huttunen
  • J Toivonen
  • Pirkko Härkönen
  • J T Soini
  • P E Hanninen
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Sidor (från-till)314-319
TidskriftJournal of Biomolecular Screening
Volym10
Utgåva nummer4
StatusPublished - 2005
PublikationskategoriForskning
Peer review utfördJa