Unique antitumour effects of L-2,4 diaminobutyric acid on cultured hepatoma cells

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric acid and N-methyl a-aminoisobutyric acid in the incubation medium did not influence the viability of hepatoma cells. We conclude that the tumour cell destructive effect of DAB was the result of a huge and unlimited uptake of DAB energized by the Na+-gradient and that this uptake was not subjected to the law of saturation kinetics. This was combined with a tumour cell energy crisis in attempts to restore the Na+-gradient.

Detaljer

Författare
  • Per-Jonas Blind
  • Alexandra Waldenström Ellervik
  • L Hafstrom
  • D Berggren
  • G Ronquist
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1245-1248
TidskriftAnticancer research
Volym23
Utgivningsnummer2B
StatusPublished - 2003
PublikationskategoriForskning
Peer review utfördJa