Unique spectral signature of human cutaneous squamous cell carcinoma by photoacoustic imaging

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with metastatic potential. To reduce reoperations due to nonradical excision, there is a need to develop a technique for identification of tumor margins preoperatively. Photoacoustic (PA) imaging is a novel imaging technology that combines the strengths of laser optics and ultrasound. Our aim was to determine the spectral signature of cSCC using PA imaging and to use this signature to visualize tumor architecture and borders. Two-dimensional PA images of 33 cSCCs and surrounding healthy skin were acquired ex vivo, using 59 excitation wavelengths from 680 to 970 nm. The spectral response of the cSCCs was compared to healthy tissue, and the difference was found to be greatest at wavelengths in the range 765 to 960 nm (P <.05). Three-dimensional PA images were constructed from spectra obtained in the y-z plane using a linear stepper motor moving along the x-plane. Spectral unmixing was then performed which provided a clear three-dimensional view of the distribution of tumor masses and their borders.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Skåne University Hospital
  • Lund University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk laboratorie- och mätteknik
  • Radiologi och bildbehandling
Originalspråkengelska
Artikelnummere201960212
TidskriftJournal of Biophotonics
StatusE-pub ahead of print - 2020 feb 12
PublikationskategoriForskning
Peer review utfördJa