Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

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Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding. / Marie, Rodolphe; Beech, Jason; Voeroes, Janos; Tegenfeldt, Jonas; Höök, Fredrik.

I: Langmuir, Vol. 22, Nr. 24, 2006, s. 10103-10108.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Marie, Rodolphe ; Beech, Jason ; Voeroes, Janos ; Tegenfeldt, Jonas ; Höök, Fredrik. / Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding. I: Langmuir. 2006 ; Vol. 22, Nr. 24. s. 10103-10108.

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TY - JOUR

T1 - Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

AU - Marie, Rodolphe

AU - Beech, Jason

AU - Voeroes, Janos

AU - Tegenfeldt, Jonas

AU - Höök, Fredrik

PY - 2006

Y1 - 2006

N2 - By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.

AB - By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.

U2 - 10.1021/la060198m

DO - 10.1021/la060198m

M3 - Article

VL - 22

SP - 10103

EP - 10108

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 24

ER -