Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper

Nyckelord

Originalspråkengelska
Titel på värdpublikationHuman Embryonic and Induced Pluripotent Stem Cells
Undertitel på gästpublikationPart of the series Springer Protocols Handbooks
RedaktörerKaiming Ye, Sha Jin
FörlagSpringer
Sidor465-480
ISBN (elektroniskt)978-1-61779-267-0
ISBN (tryckt)978-1-61779-266-3
StatusPublished - 2011 jul 30
PublikationskategoriForskning
Peer review utfördJa

Nedladdningar

Ingen tillgänglig data