Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence

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Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence. / Houston, K.; McKim, S. M.; Comadran, J.; Bonar, N.; Druka, I.; Uzrek, N.; Cirillo, E.; Guzy-Wrobelska, J.; Collins, N. C.; Halpin, C.; Hansson, Mats; Dockter, C.; Druka, A.; Waugh, R.

I: Proceedings of the National Academy of Sciences, Vol. 110, Nr. 41, 2013, s. 16675-16680.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Houston, K, McKim, SM, Comadran, J, Bonar, N, Druka, I, Uzrek, N, Cirillo, E, Guzy-Wrobelska, J, Collins, NC, Halpin, C, Hansson, M, Dockter, C, Druka, A & Waugh, R 2013, 'Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence', Proceedings of the National Academy of Sciences, vol. 110, nr. 41, s. 16675-16680. https://doi.org/10.1073/pnas.1311681110

APA

CBE

Houston K, McKim SM, Comadran J, Bonar N, Druka I, Uzrek N, Cirillo E, Guzy-Wrobelska J, Collins NC, Halpin C, Hansson M, Dockter C, Druka A, Waugh R. 2013. Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence. Proceedings of the National Academy of Sciences. 110(41):16675-16680. https://doi.org/10.1073/pnas.1311681110

MLA

Vancouver

Author

Houston, K. ; McKim, S. M. ; Comadran, J. ; Bonar, N. ; Druka, I. ; Uzrek, N. ; Cirillo, E. ; Guzy-Wrobelska, J. ; Collins, N. C. ; Halpin, C. ; Hansson, Mats ; Dockter, C. ; Druka, A. ; Waugh, R. / Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence. I: Proceedings of the National Academy of Sciences. 2013 ; Vol. 110, Nr. 41. s. 16675-16680.

RIS

TY - JOUR

T1 - Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence

AU - Houston, K.

AU - McKim, S. M.

AU - Comadran, J.

AU - Bonar, N.

AU - Druka, I.

AU - Uzrek, N.

AU - Cirillo, E.

AU - Guzy-Wrobelska, J.

AU - Collins, N. C.

AU - Halpin, C.

AU - Hansson, Mats

AU - Dockter, C.

AU - Druka, A.

AU - Waugh, R.

N1 - 41

PY - 2013

Y1 - 2013

N2 - Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosage-dependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in the mRNA of an APELATA2 (AP2)-like transcription factor, HvAP2. We used genome-wide association and biparental mapping to identify HvAP2. By comparing inflorescence development and HvAP2 transcript abundance in an extreme dense-spike mutant and its nearly isogenic WT line, we show that HvAP2 turnover driven by microRNA 172 regulates the length of a critical developmental window that is required for elongation of the inflorescence internodes. Our data indicate that this heterochronic change, an altered timing of developmental events caused by specific temporal variation in the efficiency of HvAP2 turnover, leads to the striking differences in the size and shape of the barley spike.

AB - Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosage-dependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in the mRNA of an APELATA2 (AP2)-like transcription factor, HvAP2. We used genome-wide association and biparental mapping to identify HvAP2. By comparing inflorescence development and HvAP2 transcript abundance in an extreme dense-spike mutant and its nearly isogenic WT line, we show that HvAP2 turnover driven by microRNA 172 regulates the length of a critical developmental window that is required for elongation of the inflorescence internodes. Our data indicate that this heterochronic change, an altered timing of developmental events caused by specific temporal variation in the efficiency of HvAP2 turnover, leads to the striking differences in the size and shape of the barley spike.

KW - Principal Component Analysis

KW - Molecular Sequence Data

KW - Scanning

KW - Electron

KW - Microscopy

KW - MicroRNAs/genetics/metabolism

KW - Hordeum/genetics/physiology

KW - Genome-Wide Association Study

KW - Flowers/genetics/physiology/ultrastructure

KW - Base Sequence

KW - DNA Primers/genetics

KW - Real-Time Polymerase Chain Reaction

KW - Seeds/physiology

KW - Sequence Alignment

KW - Sequence Analysis

KW - DNA

KW - Transcription Factors/genetics/metabolism

KW - genome wide association scan

KW - heterochrony

U2 - 10.1073/pnas.1311681110

DO - 10.1073/pnas.1311681110

M3 - Article

VL - 110

SP - 16675

EP - 16680

JO - Proceedings of the National Academy of Sciences

T2 - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 41

ER -