Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper
Originalspråkengelska
Artikelnummer1770
TidskriftNature Communications
Volym4
StatusPublished - 2013
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Malin Åkerblom, 2014, Molecular Neurogenetics, Faculty of Medicine, Lund University. 137 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Elgstrand, E., 2013, Department of Experimental Medical Science, Lund Univeristy. 116 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Sachdeva, R., 2013, Molecular Neurogenetics, Faculty of Medicine, Lund University. 96 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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