Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.

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Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9. / Åkerblom, Malin; Sachdeva, Rohit; Quintino, Luis; Wettergren, Erika Elgstrand; Chapman, Katie; Manfre, Giuseppe; Lindvall, Olle; Lundberg, Cecilia; Jakobsson, Johan.

I: Nature Communications, Vol. 4, 1770, 2013.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.

AU - Åkerblom, Malin

AU - Sachdeva, Rohit

AU - Quintino, Luis

AU - Wettergren, Erika Elgstrand

AU - Chapman, Katie

AU - Manfre, Giuseppe

AU - Lindvall, Olle

AU - Lundberg, Cecilia

AU - Jakobsson, Johan

PY - 2013

Y1 - 2013

N2 - Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.

AB - Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.

U2 - 10.1038/ncomms2801

DO - 10.1038/ncomms2801

M3 - Article

VL - 4

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 1770

ER -