Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.

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Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model. / Kim, Hae Jong; Kazi, Julhash U.; Lee, You Ree; Nguyen, Dung H.; Lee, Hyang Bok; Shin, Jeong Hyun; Soh, Jae Won; Kim, Eun Ki.

I: International Journal of Molecular Medicine, Vol. 25, Nr. 2, 01.02.2010, s. 249-253.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Kim, Hae Jong ; Kazi, Julhash U. ; Lee, You Ree ; Nguyen, Dung H. ; Lee, Hyang Bok ; Shin, Jeong Hyun ; Soh, Jae Won ; Kim, Eun Ki. / Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model. I: International Journal of Molecular Medicine. 2010 ; Vol. 25, Nr. 2. s. 249-253.

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TY - JOUR

T1 - Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.

AU - Kim, Hae Jong

AU - Kazi, Julhash U.

AU - Lee, You Ree

AU - Nguyen, Dung H.

AU - Lee, Hyang Bok

AU - Shin, Jeong Hyun

AU - Soh, Jae Won

AU - Kim, Eun Ki

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)

PY - 2010/2/1

Y1 - 2010/2/1

N2 - Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.

AB - Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.

U2 - 10.3892/ijmm_00000337

DO - 10.3892/ijmm_00000337

M3 - Article

VL - 25

SP - 249

EP - 253

JO - International Journal of Molecular Medicine

T2 - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1791-244X

IS - 2

ER -