Vitamin B6 Vitamer concentrations in cerebrospinal fluid differ between preterm and termnewborn infants

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

BACKGROUND AND OBJECTIVE: Vitamin B6 plays a pivotal role in brain development and functioning. Differences in vitamin B6 homeostasis between preterm and term newborn infants have been reported. The authors sought to investigate whether B6 vitamers in cerebrospinal fluid (CSF) of preterm and term newborn infants are different. METHODS: B6 vitamer concentrations were determined in 69 CSF samples of 36 newborn infants (26 born preterm and 10 born term) by ultra performance liquid chromatography-tandem mass spectrometry. CSF samples, taken from a subcutaneous intraventricular reservoir, were bedside frozen and protected from light. RESULTS: Concentrations of pyridoxal (PL), pyridoxal phosphate (PLP), pyridoxic acid (PA), and pyridoxamine (PM) in preterm newborns (postmenstrual age 30-37 weeks) were at least twice as high as in older newborns (postmenstrual age ≥42 weeks). Pyridoxine and pyridoxamine phosphate concentrations were below limits of quantification in all newborns. In CSF of 2 very preterm newborns (postmenstrual age <30 weeks), significant amounts of pyridoxine were present besides high concentrations of PL, PA, and PM, whereas PLP concentrations were relatively low. B6 vitamers in CSF were positively correlated, especially PA, PLP, and PL. CONCLUSIONS: In CSF of newborn infants, PL, PLP, PA, and PM are present, and concentrations are strongly dependent on postmenstrual age. Our results indicate that vitamin B6 homeostasis in brain differs between preterm and term newborns. These results should be taken into account for diagnosis and treatment of epilepsy and vitamin B6 deficiency in newborn infants.

Detaljer

Författare
  • Monique Albersen
  • Floris Groenendaal
  • Maria Der Van Ham
  • Tom J. De Koning
  • Marjolein Bosma
  • Wouter F. Visser
  • Gepke Visser
  • Monique G.M. De Sain-van Der Velden
  • Nanda M. Verhoeven-Duif
Externa organisationer
  • University Medical Center Utrecht
  • Wilhelmina Children’s Hospital
Forskningsområden

Nyckelord

Originalspråkengelska
TidskriftPediatrics
Volym130
Utgåva nummer1
StatusPublished - 2012 jul 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa