Whole gene family expression and drought stress regulation of aquaporins

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Whole gene family expression and drought stress regulation of aquaporins. / Alexandersson, Erik; Fraysse, Laure; Sjövall Larsen, Sara; Gustavsson, Sofia; Agemark, Maria; Karlsson, Maria; Johanson, Urban; Kjellbom, Per.

I: Plant Molecular Biology, Vol. 59, Nr. 3, 2005, s. 469-484.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Alexandersson, E, Fraysse, L, Sjövall Larsen, S, Gustavsson, S, Agemark, M, Karlsson, M, Johanson, U & Kjellbom, P 2005, 'Whole gene family expression and drought stress regulation of aquaporins', Plant Molecular Biology, vol. 59, nr. 3, s. 469-484. https://doi.org/10.1007/s11103-005-0352-1

APA

Alexandersson, E., Fraysse, L., Sjövall Larsen, S., Gustavsson, S., Agemark, M., Karlsson, M., Johanson, U., & Kjellbom, P. (2005). Whole gene family expression and drought stress regulation of aquaporins. Plant Molecular Biology, 59(3), 469-484. https://doi.org/10.1007/s11103-005-0352-1

CBE

Alexandersson E, Fraysse L, Sjövall Larsen S, Gustavsson S, Agemark M, Karlsson M, Johanson U, Kjellbom P. 2005. Whole gene family expression and drought stress regulation of aquaporins. Plant Molecular Biology. 59(3):469-484. https://doi.org/10.1007/s11103-005-0352-1

MLA

Vancouver

Alexandersson E, Fraysse L, Sjövall Larsen S, Gustavsson S, Agemark M, Karlsson M et al. Whole gene family expression and drought stress regulation of aquaporins. Plant Molecular Biology. 2005;59(3):469-484. https://doi.org/10.1007/s11103-005-0352-1

Author

Alexandersson, Erik ; Fraysse, Laure ; Sjövall Larsen, Sara ; Gustavsson, Sofia ; Agemark, Maria ; Karlsson, Maria ; Johanson, Urban ; Kjellbom, Per. / Whole gene family expression and drought stress regulation of aquaporins. I: Plant Molecular Biology. 2005 ; Vol. 59, Nr. 3. s. 469-484.

RIS

TY - JOUR

T1 - Whole gene family expression and drought stress regulation of aquaporins

AU - Alexandersson, Erik

AU - Fraysse, Laure

AU - Sjövall Larsen, Sara

AU - Gustavsson, Sofia

AU - Agemark, Maria

AU - Karlsson, Maria

AU - Johanson, Urban

AU - Kjellbom, Per

PY - 2005

Y1 - 2005

N2 - Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.

AB - Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.

U2 - 10.1007/s11103-005-0352-1

DO - 10.1007/s11103-005-0352-1

M3 - Article

C2 - 16235111

VL - 59

SP - 469

EP - 484

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 1573-5028

IS - 3

ER -