WONOEP appraisal: Optogenetic tools to suppress seizures and explore the mechanisms of epileptogenesis

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WONOEP appraisal: Optogenetic tools to suppress seizures and explore the mechanisms of epileptogenesis. / Ritter, Laura Mantoan; Golshani, Peyman; Takahashi, Koji; Dufour, Suzie; Valiante, Taufik; Kokaia, Merab.

I: Epilepsia, Vol. 55, Nr. 11, 2014, s. 1693-1702.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Ritter, LM, Golshani, P, Takahashi, K, Dufour, S, Valiante, T & Kokaia, M 2014, 'WONOEP appraisal: Optogenetic tools to suppress seizures and explore the mechanisms of epileptogenesis', Epilepsia, vol. 55, nr. 11, s. 1693-1702. https://doi.org/10.1111/epi.12804

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Ritter, Laura Mantoan ; Golshani, Peyman ; Takahashi, Koji ; Dufour, Suzie ; Valiante, Taufik ; Kokaia, Merab. / WONOEP appraisal: Optogenetic tools to suppress seizures and explore the mechanisms of epileptogenesis. I: Epilepsia. 2014 ; Vol. 55, Nr. 11. s. 1693-1702.

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TY - JOUR

T1 - WONOEP appraisal: Optogenetic tools to suppress seizures and explore the mechanisms of epileptogenesis

AU - Ritter, Laura Mantoan

AU - Golshani, Peyman

AU - Takahashi, Koji

AU - Dufour, Suzie

AU - Valiante, Taufik

AU - Kokaia, Merab

PY - 2014

Y1 - 2014

N2 - Optogenetics is a novel technology that combines optics and genetics by optical control of microbial opsins, targeted to living cell membranes. The versatility and the electrophysiologic characteristics of the light-sensitive ion-channels channelrhodopsin-2 (ChR2), halorhodopsin (NpHR), and the light-sensitive proton pump archaerhodopsin-3 (Arch) make these optogenetic tools potent candidates in controlling neuronal firing in models of epilepsy and in providing insights into the physiology and pathology of neuronal network organization and synchronization. Opsins allow selective activation of excitatory neurons and inhibitory interneurons, or subclasses of interneurons, to study their activity patterns in distinct brain-states in vivo and to dissect their role in generation of synchrony and seizures. The influence of gliotransmission on epileptic network function is another topic of great interest that can be further explored by using light-activated Gq protein-coupled opsins for selective activation of astrocytes. The ever-growing optogenetic toolbox can also be combined with emerging techniques that have greatly expanded our ability to record specific subtypes of cortical and hippocampal neurons in awake behaving animals such as juxtacellular recording and two-photon guided whole-cell recording, to identify the specific subtypes of neurons that are altered in epileptic networks. Finally, optogenetic tools allow rapid and reversible suppression of epileptic electroencephalography (EEG) activity upon photoactivation. This review outlines the most recent advances achieved with optogenetic techniques in the field of epilepsy by summarizing the presentations contributed to the 13th ILAE WONOEP meeting held in the Laurentian Mountains, Quebec, in June 2013.

AB - Optogenetics is a novel technology that combines optics and genetics by optical control of microbial opsins, targeted to living cell membranes. The versatility and the electrophysiologic characteristics of the light-sensitive ion-channels channelrhodopsin-2 (ChR2), halorhodopsin (NpHR), and the light-sensitive proton pump archaerhodopsin-3 (Arch) make these optogenetic tools potent candidates in controlling neuronal firing in models of epilepsy and in providing insights into the physiology and pathology of neuronal network organization and synchronization. Opsins allow selective activation of excitatory neurons and inhibitory interneurons, or subclasses of interneurons, to study their activity patterns in distinct brain-states in vivo and to dissect their role in generation of synchrony and seizures. The influence of gliotransmission on epileptic network function is another topic of great interest that can be further explored by using light-activated Gq protein-coupled opsins for selective activation of astrocytes. The ever-growing optogenetic toolbox can also be combined with emerging techniques that have greatly expanded our ability to record specific subtypes of cortical and hippocampal neurons in awake behaving animals such as juxtacellular recording and two-photon guided whole-cell recording, to identify the specific subtypes of neurons that are altered in epileptic networks. Finally, optogenetic tools allow rapid and reversible suppression of epileptic electroencephalography (EEG) activity upon photoactivation. This review outlines the most recent advances achieved with optogenetic techniques in the field of epilepsy by summarizing the presentations contributed to the 13th ILAE WONOEP meeting held in the Laurentian Mountains, Quebec, in June 2013.

KW - Epilepsy

KW - Interneurons

KW - Gliotransmission

KW - Optic inhibition

KW - Halorhodopsin

KW - Channelrhodopsin

KW - Seizure detection

U2 - 10.1111/epi.12804

DO - 10.1111/epi.12804

M3 - Article

VL - 55

SP - 1693

EP - 1702

JO - Epilepsia

T2 - Epilepsia

JF - Epilepsia

SN - 0013-9580

IS - 11

ER -