Xyloside-primed chondroitin sulfate/dermatan sulfate from breast carcinoma cells with a defined disaccharide composition has cytotoxic effects in vitro

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We have previously reported that the xyloside 2-(6-hydroxynaphthyl) β-D-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-D-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo. Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking. In this study, we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate (CS/DS) GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells. The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate (HS) GAGs. In contrast, neither the CS/DS nor the HS derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells. These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed considerably between the two cell lines, but was similar when the GAGs were derived from the same cell line. To our knowledge, this is the first report on cytotoxic effects mediated by CS/DS.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Läkemedelskemi
  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)14871-14882
Antal sidor12
TidskriftJournal of Biological Chemistry
Volym291
Utgivningsnummer28
Tidigt onlinedatum2016 maj 12
StatusPublished - 2016 jul
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Persson, A., 2017, Lund: Lund University, Faculty of Medicine. 93 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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