Xylosyl transfer to the core protein precursor of the rat chondrosarcoma proteoglycan

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Xylosyl transfer to the core protein precursor of the rat chondrosarcoma proteoglycan. / Lohmander, Stefan; Shinomura, T.; Hascall, V. C.; Kimura, J. H.

I: Journal of Biological Chemistry, Vol. 264, Nr. 31, 1989, s. 18775-18780.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Lohmander, S, Shinomura, T, Hascall, VC & Kimura, JH 1989, 'Xylosyl transfer to the core protein precursor of the rat chondrosarcoma proteoglycan', Journal of Biological Chemistry, vol. 264, nr. 31, s. 18775-18780.

APA

CBE

MLA

Vancouver

Author

Lohmander, Stefan ; Shinomura, T. ; Hascall, V. C. ; Kimura, J. H. / Xylosyl transfer to the core protein precursor of the rat chondrosarcoma proteoglycan. I: Journal of Biological Chemistry. 1989 ; Vol. 264, Nr. 31. s. 18775-18780.

RIS

TY - JOUR

T1 - Xylosyl transfer to the core protein precursor of the rat chondrosarcoma proteoglycan

AU - Lohmander, Stefan

AU - Shinomura, T.

AU - Hascall, V. C.

AU - Kimura, J. H.

PY - 1989

Y1 - 1989

N2 - Rat chondrosarcoma chondrocytes were labeled with [3H]serine or [3H]mannose as a precursor. Intracellular proteoglycan core protein precursor was purified from cell lysates by immunoprecipitation with polyclonal antibodies against the hyaluronic acid-binding region, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The core precursor was eluted from the gels and treated with alkaline borohydride in order to convert serine residues substituted with xylose or N-acetylgalactosamine to alanine (or with alkaline sulfite to convert them to cysteic acid). After acid hydrolysis, the proportions of labeled serine and alanine (or cysteic acid) were determined by high performance liquid chromatography, and the results were compared with those obtained for the completed proteoglycan molecules isolated from the same cultures. In the completed proteoglycans, about 55% of the serine residues were substituted with xylose or N-acetylgalactosamine, while the corresponding figure for the intracellular precursor molecules was less than 5%. These results indicate, in agreement with our previous kinetic data, that the major part of the xylosyl transfer to the chondrosarcoma proteoglycan core protein precursor must occur late in the processing sequence, i.e. after about 85% of its intracellular lifetime and no more than 7 min before the addition of the rest of the chondroitin sulfate chain. The ratio of [3H]mannose to [3H]fucose in the core precursor was about 19, while that for the complete proteoglycan was about 2. This indicates the presence of high mannose, N-linked oligosaccharides on the core protein precursor which are converted to the complex forms on the completed proteoglycan. These data provide further support that the core precursor resides mainly in the pre-Golgi compartment and that xylosylation occurs mainly in a Golgi compartment.

AB - Rat chondrosarcoma chondrocytes were labeled with [3H]serine or [3H]mannose as a precursor. Intracellular proteoglycan core protein precursor was purified from cell lysates by immunoprecipitation with polyclonal antibodies against the hyaluronic acid-binding region, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The core precursor was eluted from the gels and treated with alkaline borohydride in order to convert serine residues substituted with xylose or N-acetylgalactosamine to alanine (or with alkaline sulfite to convert them to cysteic acid). After acid hydrolysis, the proportions of labeled serine and alanine (or cysteic acid) were determined by high performance liquid chromatography, and the results were compared with those obtained for the completed proteoglycan molecules isolated from the same cultures. In the completed proteoglycans, about 55% of the serine residues were substituted with xylose or N-acetylgalactosamine, while the corresponding figure for the intracellular precursor molecules was less than 5%. These results indicate, in agreement with our previous kinetic data, that the major part of the xylosyl transfer to the chondrosarcoma proteoglycan core protein precursor must occur late in the processing sequence, i.e. after about 85% of its intracellular lifetime and no more than 7 min before the addition of the rest of the chondroitin sulfate chain. The ratio of [3H]mannose to [3H]fucose in the core precursor was about 19, while that for the complete proteoglycan was about 2. This indicates the presence of high mannose, N-linked oligosaccharides on the core protein precursor which are converted to the complex forms on the completed proteoglycan. These data provide further support that the core precursor resides mainly in the pre-Golgi compartment and that xylosylation occurs mainly in a Golgi compartment.

UR - http://www.scopus.com/inward/record.url?scp=0024414803&partnerID=8YFLogxK

M3 - Article

C2 - 2509468

AN - SCOPUS:0024414803

VL - 264

SP - 18775

EP - 18780

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 1083-351X

IS - 31

ER -